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Award Abstract #0103414
NER: Fluorescence Microscopy with 5 nm Resolution for Imaging the Membrane of a Living Cell
| NSF Org: |
CBET
Division of Chemical, Bioengineering, Environmental, and Transport Systems
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| Initial Amendment Date: |
June 21, 2001 |
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| Latest Amendment Date: |
June 21, 2001 |
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| Award Number: |
0103414 |
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| Award Instrument: |
Standard Grant |
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| Program Manager: |
RICHARD N. SMITH
CBET Division of Chemical, Bioengineering, Environmental, and Transport Systems
ENG Directorate for Engineering
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| Start Date: |
July 1, 2001 |
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| Expires: |
June 30, 2002 (Estimated) |
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| Awarded Amount to Date: |
$99367 |
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| Investigator(s): |
Jeffrey White white5@uiuc.edu (Principal Investigator)
Sandy Helman (Co-Principal Investigator)
Munir Nayfeh (Co-Principal Investigator)
Taekjip Ha (Co-Principal Investigator)
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| Sponsor: |
University of Illinois at Urbana-Champaign
SUITE A
CHAMPAIGN, IL 61820 217/333-2187
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| NSF Program(s): |
THERMAL TRANSPORT PROCESSES
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| Field Application(s): |
0308000 Industrial Technology
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| Program Reference Code(s): |
OTHR, 1676, 0000
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| Program Element Code(s): |
1406
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ABSTRACT
Abstract
This proposal was received in response to NSE, NSF-0019. A Forster transfer microscope (FTM) will be developed to allow in vivo imaging of living cells with 5 nm resolution. The unprecedented resolution will be achieved through non-radiative Forster energy transfer between a scanning probe containing a silicon nanoparticle and a sample tagged with dye molecules. The FTM will exploit two recent developments in nanotechnology: (i) the discovery, at the University of Illinois, of a technique for fabricating 1 nm silicon nanoparticles with excellent size uniformity and fluorescence efficiency, and (ii) the development of a combined atomic force microscope / near-field scanning optical microscope (AF-NSOM) with 50 nm optical resolution and 1 nm height resolution.
The FTM will be used to solve a long-standing question about how hormones and drugs interact with epithelial membranes: "Is ion transport modulated by movement of ion channels to and from the apical membrane (vesical trafficking), or by changes in their activation state?" As cultured epithelial membranes become a more widely used biomaterial, e.g. skin for burn victims, an understanding of cell dynamics on the 100 nm scale and below will be invaluable.
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