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Award Abstract #0110154
Activation-Tagged and Deletion Mutants and cDNA Microarrays for Functional Genomics of Rice
| NSF Org: |
IOS
Division of Integrative Organismal Systems
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| Initial Amendment Date: |
September 26, 2001 |
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| Latest Amendment Date: |
February 11, 2003 |
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| Award Number: |
0110154 |
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| Award Instrument: |
Standard Grant |
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| Program Manager: |
Jane Silverthorne
IOS Division of Integrative Organismal Systems
BIO Directorate for Biological Sciences
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| Start Date: |
September 1, 2001 |
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| Expires: |
August 31, 2003 (Estimated) |
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| Awarded Amount to Date: |
$534645 |
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| Investigator(s): |
Jan Leach jan.leach@colostate.edu (Principal Investigator)
Patrick Schnable (Co-Principal Investigator)
Pamela Ronald (Co-Principal Investigator)
Hei Leung (Co-Principal Investigator)
Guo-Liang Wang (Co-Principal Investigator)
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| Sponsor: |
Kansas State University
2 FAIRCHILD HALL
MANHATTAN, KS 66506 785/532-6804
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| NSF Program(s): |
PLANT GENOME RESEARCH PROJECT
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| Field Application(s): |
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| Program Reference Code(s): |
BIOT, 9251, 9109
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| Program Element Code(s): |
1329
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ABSTRACT
Rice genome sequencing projects are defining thousands of new rice genes. However, the critical tools required to determine the functions of these genes are not yet available to public-sector researchers. To remedy this deficiency, novel approaches to generate collections of insertion mutants and to detect mutated genes in a complementary set of rice deletion mutants will be developed. The first objective is to develop and test vectors for creating activation-tagged rice mutants using the Ac/Ds system. Activation tagging allows the identification of gene knockout mutants and mutants exhibiting gene over-expression. The vectors will contain chemical-induced promoters that will allow the controlled transposition of the Ds element. The second objective is to develop and optimize a high throughput protocol for the detection of mutated genes in an existing deletion mutant collection. The strategy is to use polymerase chain reaction (PCR) to detect the mutated genes rapidly in pools of DNA from many mutants. The rapid detection of mutated genes by this protocol will allow the identification of mutants with deletions in specific genes of interest. The international dimension of the project will enrich the training experience of the postdoctoral fellows and extend the impact of the research.
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