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Award Abstract #0211872
Functional Genomics of Host-Virus Interactions
| NSF Org: |
IOS
Division of Integrative Organismal Systems
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| Initial Amendment Date: |
July 29, 2002 |
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| Latest Amendment Date: |
January 10, 2008 |
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| Award Number: |
0211872 |
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| Award Instrument: |
Continuing grant |
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| Program Manager: |
Diane Jofuku Okamuro
IOS Division of Integrative Organismal Systems
BIO Directorate for Biological Sciences
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| Start Date: |
August 1, 2002 |
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| Expires: |
April 30, 2008 (Estimated) |
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| Awarded Amount to Date: |
$3363177 |
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| Investigator(s): |
Savithramma Dinesh-Kumar savithramma.dinesh-kumar@yale.edu (Principal Investigator)
Hongyu Zhao (Co-Principal Investigator)
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| Sponsor: |
Yale University
P.O. Box 208337
NEW HAVEN, CT 06520 203/432-2460
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| NSF Program(s): |
PLANT GENOME RESEARCH PROJECT
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| Field Application(s): |
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| Program Reference Code(s): |
BIOT, 9109
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| Program Element Code(s): |
1329
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ABSTRACT
The relationship between eukaryotic viruses and their hosts are characteristic of most
host-pathogen relationships that have co-evolved. The outcomes of virus-host interactions are genetically pre-determined. To produce disease, viruses must enter the host, multiply locally in host tissues, spread from the site of entry, and overcome or evade host immune responses. Plants have evolved various anti-viral defense strategies to clear viral infection. On the other hand, viruses have evolved counter-defense strategies. Therefore, it is important to understand molecular mechanisms of how viruses evade the host's antiviral defenses.
A Tobacco Rattle Virus-based virus induced gene silencing (VIGS) system will be used to identify suppressors of viral resistance and susceptibility factors. In addition, a functional proteomics approach will be used to study virus-host interactions. The knowledge gained from these studies will help to combat infectious plant diseases. Protection of crops from disease can significantly improve agricultural production. Application of a plant's own defense mechanism can lead to more effective protection against plant pathogens. Control of pathogen-induced diseases using cellular genes that function in the disease resistance signaling pathway may provide tremendous agricultural benefits and serve the environment by offering an alternative to pesticide use to prevent disease. Tools and information developed in this project will be made available to the scientific community. These resources will assist in the efforts to improve economically important plants like tomato, potato and pepper.
Deliverables
Available now: pTRV1, pTRV2, pTRV2-GATEWAY, pTRV-NbPDS, pTRV-tomPDS
VIGS system. Contact savithramma.dinesh-kumar@yale.edu
Available by 9/03: 250-300 sequence verified pTRV2-tomato EST clones
1000-1500 sequence verified pTRV2-Nb-cDNA Agrobacterium
TMV-TAP vector
PUBLICATIONS PRODUCED AS A RESULT OF THIS RESEARCH
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(Showing: 1 - 13 of 13)
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Burch-Smith, T.M., Anderson, J.C., Martin, G.B., and Dinesh-Kumar, S.P. "Applications and advantages of virus induced gene silencing (VIGS) for gene function studies in plants.," Plant Journal, v.39, 2004, p. 734.
Burch-Smith, T.M., Schiff, M., Liu, Y., and Dinesh-Kumar, S.P. "Efficient virus induced gene silencing in Arabidopsis thaliana.," Plant Physiology, v.142, 2006, p. 21-27.
Caplan, J. C., Padmanabhan, M., and Dinesh-Kumar, S.P.. "Plant NB-LRR immune receptors: From recognition to transcriptional reprogramming.," Cell Host & Microbe, v.3, 2008, p. 126.
Caplan, J., and Dinesh-Kumar, S.P.. "Using viral vectors to silence endogenous genes.," Current Protocols in Microbiology, v.I6I.6.1, 2006.
Dinesh-Kumar, S. P., Anandalakshmi, R., Marathe, R., Schiff, M., and Liu, Y.. "Virus-induced gene silencing.," Methods in Molecular Biology, v.236, 2003, p. 287.
Dong, Y., Burch-Smith, T., Liu, Y., and Dinesh-Kumar, S.P.. "A ligation-independent cloning TRV vector for high-throughput virus induced gene silencing identifies roles for NbMADS4-1 and ?2 in floral development.," Plant Physiology, v.145, 2007, p. 1161.
Ekengren, S. K., Liu, Y., Schiff, M., Dinesh-Kumar, S. P., and Martin, G. B.. "Two MAPK cascades, NPR1, and TGA transcription factors play a role in Pto-mediated disease resistance in tomato.," Plant Journal, v.36, 2003, p. 905.
Liu, Y., Nakayama, N., Schiff, M., Litt, A., Irish, V.F., Dinesh-Kumar, S.P.. "Virus-induced gene silencing of a DEFICIENS ortholog in Nicotiana benthamiana.," Plant Molecular Biology, v.54, 2004, p. 701.
Liu, Y., Schiff, M., and Dinesh-Kumar, S. P.. "Involvement of MEK1 MAPKK, NTF6 MAPK, WRKY/MYB transcription factors, COI1 and CTR1 in N-mediated resistance to tobacco mosaic virus.," Plant Journal, v.38, 2004, p. 800.
Liu, Y., Schiff, M., Czymmek, K., Talloczy, Z., Levine, B., and Dinesh-Kumar, S.P.. "Autophagy regulates programmed cell death during the plant innate immune response.," Cell, v.121, 2005, p. 567.
Rubio, V., Shen, Y., Saijo, Y., Liu, Y., Gusmaroli, G., Dinesh-Kumar, S.P., and Deng, X.W.. "An alternative tandem affinity purification strategy applied to Arabidopsis protein complex isolation.," Plant Journal, v.41, 2005, p. 767.
Soosaar, J.L.M., Burch-Smith, T.M., and Dinesh-Kumar, S.P.. "Mechanisms of Plant Resistance to Viruses.," Nature Reviews Microbiology, v.3, 2005, p. 789.
Zhu, X., and Dinesh-Kumar, S.P.. "Virus induced gene silencing as a tool to identify host genes affecting viral pathogenicity.," Methods in Molecular Biology: Plant Virology Protocols, v.451, 2008, p. 641.
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