Award Abstract #0211924
Insertional Mutagenesis Tools for Functional Genomics in Rice

NSF Org:	DBI Division of Biological Infrastructure
Initial Amendment Date:	August 15, 2002
Latest Amendment Date:	July 15, 2005
Award Number:	0211924
Award Instrument:	Standard Grant
Program Manager:	Diane Jofuku Okamuro DBI Division of Biological Infrastructure BIO Directorate for Biological Sciences
Start Date:	September 1, 2002
Expires:	November 30, 2006 (Estimated)
Awarded Amount to Date:	$856532
Investigator(s):	Venkatesan Sundaresan sundar@ucdavis.edu (Principal Investigator)
Sponsor:	University of California-Davis OR/Sponsored Programs Davis, CA 95618 530/754-7000
NSF Program(s):	PLANT GENOME RESEARCH PROJECT
Field Application(s):
Program Reference Code(s):	BIOT, 9251, 9109, 1228
Program Element Code(s):	1329

ABSTRACT

Award Abstract

Rice, the second plant genome to be completely sequenced, has a relatively small genome of about 430 million base pairs. While rice is also amenable to genetic studies and transformation and is a model system for genome studies of the cereals, it is also an important crop. Currently, there are few tools for identifying the functions of sequenced genes for rice that are readily available to the research community.

One of the most effective approaches to deciphering gene functions is through gene disruption by DNA insertions. In this project, we will develop efficient transposon tagging strategies for large-scale insertion mutagenesis in rice. Stable insertion lines will be generated containing random insertions of the maize En/Spm transposons in japonica rice cv. Nipponbare, which is the cultivar selected for the International Rice Genome Sequencing Project. A publicly accessible database for reverse genetics containing sequences of DNA flanking insertions will be established, and the sequences will be linked to the rice genome database and the rice genome physical map. Overall, this project should develop a valuable resource for functional genomics in rice, as well as other cereals.

PUBLICATIONS PRODUCED AS A RESULT OF THIS RESEARCH

Hirochika H, Guiderdoni E, An G, Hsing YI, Eun MY, Han CD, Upadhyaya N, Ramachandran S, Zhang Q, Pereira A, Sundaresan V, Leung H.. "Rice mutant resources for gene discovery," Plant Molecular Biology, v.54, 2004, p. 325.

Kumar, C.S., Wing, R.A., Sundaresan, V.. "Efficient insertional mutagenesis in rice using the maize En/Spm elements," The Plant Journal (2005) 44, 879?892, v.44, 2005, p. 879.

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