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Award Abstract #0211935
From Proplastid to Chloroplast: Understanding Plastid Differentiation in Maize through Microarray and Proteome Analysis
| NSF Org: |
IOS
Division of Integrative Organismal Systems
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| Initial Amendment Date: |
September 11, 2002 |
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| Latest Amendment Date: |
July 31, 2006 |
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| Award Number: |
0211935 |
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| Award Instrument: |
Continuing grant |
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| Program Manager: |
Diane Jofuku Okamuro
IOS Division of Integrative Organismal Systems
BIO Directorate for Biological Sciences
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| Start Date: |
September 1, 2002 |
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| Expires: |
August 31, 2008 (Estimated) |
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| Awarded Amount to Date: |
$3846527 |
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| Investigator(s): |
Klaas vanWijk kv35@cornell.edu (Principal Investigator)
David Stern (Co-Principal Investigator)
Thomas Brutnell (Co-Principal Investigator)
Thomas Clemente (Co-Principal Investigator)
Lori Allison (Former Co-Principal Investigator)
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| Sponsor: |
Cornell University - State
373 Pine Tree Road
Ithica, NY 14850 607/255-5014
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| NSF Program(s): |
PLANT GENOME RESEARCH PROJECT
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| Field Application(s): |
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| Program Reference Code(s): |
BIOT, 9232, 9184, 9109
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| Program Element Code(s): |
1329
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ABSTRACT
Although best known for their role in photosynthesis, plastids perform several essential functions, which include synthesis of hormones, amino acids, fatty acids, lipids, vitamins, nucleotides and secondary metabolites. Despite their essential role, little is known of the developmental signals that help drive the process of plastid differentiation.. To address this important agricultural and biological problem, we will take advantage of new tools that have been developed for genomic and proteomic studies. The availability of extensive plant genome sequences has enabled us to identify a set of approximately 1500 maize EST sequences that encode chloroplast targeted proteins. Through array profiling, we will examine the expression of these genes in the two photosynthetic cell types in maize, the bundle sheath and mesophyll. In parallel, we will initiate a broad survey of the estimated 3,000 proteins predicted to accumulate in plastids using highly sensitive mass spectroscopy techniques. The bioinformatic integration of proteomic and expression profiling data will provide a detailed picture of transcription and translational control mechanisms utilized by the maize photosynthetic plastids. Finally, transgenic plants carrying bundle sheath and mesophyll cell-specific markers will be constructed to help monitor the differentiation process. These GFP constructs will permit a detailed analysis of bundle sheath and mesophyll cell biology and facilitate the analysis of a series of maize plastid mutants. Several results are expected from this project, such as an improved understanding of the biochemical functions of the different plastid types and novel insights into the differentiation process. As several chloroplast functions are directly related to the agronomic performance of the maize plant, these studies will greatly improve our understanding and ability to engineer this important crop plant.
PUBLICATIONS PRODUCED AS A RESULT OF THIS RESEARCH
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(Showing: 1 - 10 of 11)
(Showing: 1 - 11 of 11)
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Cahoon, A.B., Takacs, E.M., Sharpe, R.M., and Stern, D.B.. "Nuclear, chloroplast, and mitochondrial transcript abundance along a maize leaf developmental gradient.," Plant Mol Biol., v.on-line, 2007.
Cahoon, A.B., Takacs, E.M., Sharpe, R.M., and Stern, D.B.. "Nuclear, chloroplast, and mitochondrial transcript abundance along a maize leaf developmental gradient.," Plant Mol Biol., v.66, 2007, p. 33.
Covshoff, S., Majeran, W., Liu, P., Kolkman, J.M., van Wijk, K.J., and Brutnell, T.P. "Deregulation of maize c4 photosynthetic development in a mesophyll cell-defective mutant.," Plant Physiology, v.146, 2008, p. 1469.
Irihimovitch V and Stern DB. "The sulfur acclimation SAC3 kinase is required for chloroplast transcriptional repression under sulfur limitation in Chlamydomonas reinhardtii.," Proc. Natl. Acad. Sci. USA, v.103, 2006, p. 9711.
Majeran, W., Cai, Y., Sun, Q., van Wijk, K.J.. "Functional differentiation of Bundle Sheath and Mesophyll maize chloroplasts determined by Comparative proteomics," The Plant Cell, v.17, 2005, p. 3111.
Majeran, W., Zybailov, B., Ytterberg, A.J., Dunsmore, J., Sun, Q., and van Wijk, K.J.. "Consequences of C4 differentiation for chloroplast membrane proteomes in maize mesophyll and bundle sheath cells.," Mol Cell Proteomics, v.7, 2008, p. 1609.
Sawers, R.J., Farmer, P.R., Moffett, P., and Brutnell, T.P.. "In planta transient expression as a system for genetic and biochemical analyses of chlorophyll biosynthesis," Plant Methods, v.2, 2006, p. 15.
Sawers, R.J., Liu, P., Anufrikova, K., Hwang, J.T., and Brutnell, T.P.. "A multi-treatment experimental system to examine photosynthetic differentiation in the maize leaf.," BMC Genomics, v.8, 2007, p. 12.
Sawers, R.J.H., J. Viney, P. R. Farmer, R.R. Bussey*, G. Olsefski*, K. Anufrikova, C. N. Hunter and T. P. Brutnell. "The maize Oil yellow1 (Oy1) gene encodes the I subunit of magnesium chelatase," Plant Mol Biol, v.60, 2006, p. 95.
Sun, Q., Zybailov, B., Majeran, W., Friso, G., Olinares, P.D., and van Wijk, K.J.. "PPDB, the Plant Proteomics Database at Cornel," Nucleic Acids Res., v.online, 2008.
Wostrikoff, K., and Stern, D.. "Rubisco large-subunit translation is autoregulated in response to its assembly state in tobacco chloroplasts.," Proc Natl Acad Sci U S A, v.104, 2007, p. 6466.
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