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Award Abstract #0217552
Identification and Characterization of Cell Wall Mutants in Maize and Arabidipsis using Novel Spectroscopies
| NSF Org: |
IOS
Division of Integrative Organismal Systems
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| Initial Amendment Date: |
September 20, 2002 |
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| Latest Amendment Date: |
August 29, 2008 |
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| Award Number: |
0217552 |
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| Award Instrument: |
Cooperative Agreement |
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| Program Manager: |
Diane Jofuku Okamuro
IOS Division of Integrative Organismal Systems
BIO Directorate for Biological Sciences
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| Start Date: |
September 1, 2002 |
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| Expires: |
August 31, 2008 (Estimated) |
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| Awarded Amount to Date: |
$6338840 |
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| Investigator(s): |
Nicholas Carpita carpita@purdue.edu (Principal Investigator)
Karen Koch (Co-Principal Investigator)
Wolf-Dieter Reiter (Co-Principal Investigator)
Patrick Krysan (Co-Principal Investigator)
Steven Thomas (Co-Principal Investigator)
Sara Patterson (Former Co-Principal Investigator)
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| Sponsor: |
Purdue University
Young Hall
West Lafayette, IN 47907 765/494-4600
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| NSF Program(s): |
PLANT GENOME RESEARCH PROJECT
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| Field Application(s): |
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| Program Reference Code(s): |
BIOT, 9251, 9232, 9109, 7462, 1228
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| Program Element Code(s): |
1329
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ABSTRACT
Two methods, discriminant analysis of Fourier transform mid-range and near infrared spectra, have been developed as high throughput approaches to identifying mutations in plant cell wall polysaccharide components and wall architecture.
These methods will be employed to identify in insertion tagged populations of maize and Arabidopsis a broad range of mutants involved in the biogenesis and dynamic alteration of plant cell wall architecture during growth and development. A systematic protocol using biochemical, spectroscopic, and imaging methods has been devised to categorize defects in wall structure and architecture related to development into one of six stages of wall biogenesis or disassembly. Maize lines carrying Mu-insertions and Arabidopsis lines carrying T-DNA tags will be used as primary material for both forward and reverse screens.
A major practical goal of the project is to generate plants with genetically defined variation in wall composition and architecture that will permit assessment of modifications on wall properties and plant development. Since cell walls are an enormously important source of industrial raw material, we anticipate that several of the genes we identify and characterize, as well as several of the plants with genetically defined alterations, will be of economic importance. As the heritability of the mutations is confirmed and information on the possible function of the genes identified is obtained, the plant biology community will be informed of them through a web site that has been created as a repository for all cell-wall related genomics. Maize and Arabidopsis stock centers will be provided with all materials to dispense to the community at large.
PUBLICATIONS PRODUCED AS A RESULT OF THIS RESEARCH
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Dean, G.H., Zheng, H., Tewari, J., Huang, J., Young, D.S., Hwang, Y.T., Western, T.L., Carpita, N.C., McCann, M.C., Mansfield, S.D., Haughn, G.W.. "The Arabidopsis MUM2 gene encodes a β-galactosidase required for the production of seed coat mucilage with correct hydration properties.," Plant Cell, v.19, 2007, p. 4007.
del Campillo, E., Mohammed, A., Crawford, D., Patterson, S. E.. "Root cap specific expression of an endo-?Ò-1,4-D-glucanase (cellulase): A new marker to study root development in Arabidopsis.," Plant Mol. Biol., v.56, 2004, p. 309.
del Campillo, E., Mohammed, A., Crawford, D., Patterson, S. E.. "Root cap specific expression of an endo-beta-1,4-D-glucanase (cellulase): A new marker to study root development in Arabidopsis.," Plant Molecular Biology, v.56, 2004, p. 309.
Eveland A.L., D.R. McCarty, and K.E. Koch.. "Transcript profiling by 3?UTR sequencing resolves expression of gene families.," Plant Physiol, v.246, 2007, p. 1.
Eveland A.L., D.R. McCarty, and K.E. Koch.. "Transcript profiling by 3'UTR sequencing resolves expression of gene families.," Plant Physiology, v.146, 2008, p. 32.
Huang, L-F, P.N. Bocock, J.M. Davis, K.E. Koch.. "Regulation of invertases: A 'suite' of transcriptional and post-transcriptional mechanisms.," Functional Plant Biology, v.34, 2007, p. 499.
Huang, L-F, P.N. Bocock, J.M. Davis, K.E. Koch.. "Regulation of invertases: A ?suite? of transcriptional and post-transcriptional mechanisms.," Functional Plant Biology., v.34, 2007, p. 499.
Kohorn B., M. Kobayashi, S. Johansen, J. Riese, L-F. Huang, K. Koch, A. Dotson, N. Byers. "An Arabidopsis cell wall associated kinase required for invertase activity and cell growth.," Plant J., v.46, 2006, p. 307.
Kohorn B., M. Kobayashi, S. Johansen, J. Riese, L-F. Huang, K. Koch, A. Dotson, N. Byers. "An Arabidopsis cell wall associated kinase required for invertase activity and cell growth.," Plant Journal, v.46, 2006, p. 307.
Lao, N. T., Long, D., Kiang, S., Coupland, G., Shoue, D.A. Carpita, N.C., Kavanagh, T.A.. "Mutation of a family 8 glycosyltransferase gene alters cell wall carbohydrate composition and causes a humidity-sensitive semi-sterile dwarf phenotype in Arabidopsis.," Plant Molecular Biology, v.53, 2004, p. 647.
McCann MC, Carpita NC. "Looking for invisible phenotypes in cell wall mutants of Arabidopsis thaliana.," Plant Biosystems, v.139, 2005, p. 80.
McCann, M. C., N. C. Carpita. "Looking for invisible phenotypes in cell-wall mutants of Arabidopsis thaliana.," Plant Biosystems, v.139, 2005, p. 80.
McCann, M. C., N. C. Carpita. "Looking for invisible phenotypes in cell-wall mutants of Arabidopsis thaliana.," Plant Biosystems, v.139, 2005, p. 80.
McCann, M.C., Carpita, N.C.. "Designing the deconstruction of plant cell walls.," Current Opinions in Plant Biology, v.11, 2008, p. 314.
McCann, M.C., Defernez, M., Urbanowicz, B.R., Tewari, J.C., Langewisch, T., Olek, A., Wells, B., Wilson, R.H., Carpita N.C.. "Neural network analyses of infrared spectra for classifying cell wall architectures.," Plant Physiology, v.143, 2007, p. 1314.
Naran, R., Chen, G., Carpita, N.C.. "Novel rhamnogalacturonan I and arabinoxylan from flax mucilage.," Plant Physiology, v.148, 2008, p. 132.
O'Malley, R.O.; Walsh, B.A., Bleecker, A.B., Patterson, S.E.. "OLIGOGO: Primer design for identification of knockouts and RT PCR.," Nucl. Acids Res., 2007.
Patterson S.E., and Bleecker, A. B.. "Ethylene dependent and independent processes associated with floral organ abscission in Arabidopsis.," Plant Physiol., v.134, 2004, p. 194.
Patterson S.E., and Bleecker, A. B.. "Ethylene dependent and independent processes associated with floral organ abscission in Arabidopsis.," Plant Physiology, v.134, 2004, p. 194.
Settles, A.M., D.R. Holding, B-C Tan, S.P Latshaw, J. Liu, M. Suzuki, B. O'Brien, D. Fajardo, E. Wroclwaska, J. Lai, C. Hunter, W.T. Avigne, S.O. Peacock, J. Baier, D. Lonon, J. Messing, L.C. Hannah, K.E. Koch, P.W. Becraft, B.A. Larkins, D. "Sequence-indexed mutations in maize using the UniformMu transposon-tagging population.," BMC-Genomics, v.8, 2007, p. Art. 116.
Settles, A.M., D.R. Holding, B-C Tan, S.P Latshaw, J. Liu, M. Suzuki, B. O?Brien, D. Fajardo, E. Wroclwaska, J. Lai, C. Hunter, W.T. Avigne, S.O. Peacock, J. Baier, D. Lonon, J. Messing, L.C. Hannah, K.E. Koch, P.W. Becraft, B.A. Larkins, D. "Sequence-indexed mutations in maize using the UniformMu transposon-tagging population.," BMC-Genomics, 2007.
Sindhu, A., Langewisch, T., Olek, A., Multani, D.S., McCann, M.C., Vermerris, W., Carpita, N.C., Johal, G.. "Maize Brittle stalk2 encodes a COBRA-like protein expressed in early organ development but required for tissue flexibility at maturity.," Plant Physiology, v.145, 2007, p. 1444.
Vermerris W, Saballos A, Ejeta G, Mosier NS, Ladisch MR, Carpita NC. "Molecular breeding to enhance ethanol production from maize and sorghum stover.," Crop Sci., v.47, 2007, p. S142.
Vermerris, W., Saballos, A., Ejeta, G., Mosier, N.S., Ladisch, M.R., Carpita, N.C.. "Molecular breeding to enhance ethanol production from maize and sorghum stover.," Crop Science, v.47, 2007, p. S142.
Yong, W., B. Link, R. O?Malley, J. Tewari, C. T. Hunter III, C-A. Lu, X. Li, A. B. Bleecker, K. E. Koch, M. C. McCann, D. R. McCarty, S. E. Patterson, W-D. Reiter, C. Staiger, S. R. Thomas, W. Vermerris, N. C. Carpita.. "Genomics of plant cell wall biogenesis.," Planta, v.221, 2005, p. 747.
Yong, W., R. OMalley, B. Link, B. Binder, A. Bleecker, K. E. Koch, D. R. McCarty, S. Patterson, W-D. Reiter, C. J. Staiger, S. Thomas, W. Vermerris, M. C. McCann, N. C. Carpita. "Plant cell wall genomics," Planta, v.221, 2005, p. 747.
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