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Award Abstract #0217653
Integrative Functional Genomic Resource Development in Vitis Vinifera: Abiotic Stress and Wine Quality
| NSF Org: |
IOS
Division of Integrative Organismal Systems
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| Initial Amendment Date: |
September 19, 2002 |
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| Latest Amendment Date: |
February 26, 2008 |
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| Award Number: |
0217653 |
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| Award Instrument: |
Continuing grant |
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| Program Manager: |
Diane Jofuku Okamuro
IOS Division of Integrative Organismal Systems
BIO Directorate for Biological Sciences
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| Start Date: |
September 15, 2002 |
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| Expires: |
August 31, 2008 (Estimated) |
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| Awarded Amount to Date: |
$3732858 |
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| Investigator(s): |
Grant Cramer cramer@unr.edu (Principal Investigator)
David Schooley (Co-Principal Investigator)
John Cushman (Co-Principal Investigator)
Pedro Mendes (Co-Principal Investigator)
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| Sponsor: |
University of Nevada Reno
1664 North Virginia Street
Reno, NV 89557 775/784-4040
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| NSF Program(s): |
PLANT GENOME RESEARCH PROJECT
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| Field Application(s): |
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| Program Reference Code(s): |
BIOT, 9251, 9109
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| Program Element Code(s): |
1329
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ABSTRACT
Abiotic stress in the form of drought, salinity, and cold has a major impact on grape production and quality. Several studies have shown that water-deficit-stressed grapevines produce superior quality wine. The molecular genetic and biochemical basis for this correlation remains poorly understood. An integrative and quantitative analysis of mRNA, protein, and metabolite changes following abiotic stress imposition is required to enhance production efficiency under stress conditions and to understand the plant-derived contribution to constituents of wine quality. It will also require the customization and application of comprehensive bioinformatics systems to track and analyze changes that arise in response to abiotic stress and potentially related aroma, flavor, and color characteristics of grape juice and wine. One long-term goal of our research is to develop comprehensive genomic tools to facilitate the genetic engineering of improved abiotic stress tolerance traits in V. vinifera. The specific objectives for accomplishing this goal include: 1) extensive gene discovery through large-scale expressed sequence tag (EST) sequencing and mRNA expression profiling using oligonucleotide microarray-based expression monitoring in roots, leaves, and fruits of grapevines exposed to multiple abiotic stresses; 2) global mRNA expression profile data will be complemented by protein expression analyses using state-of-the-art proteomics methodologies; and 3) identification of specific metabolites and metabolite profiles in grapevines and fruit following abiotic stress that confer desirable aroma, flavor and color quality characteristics and improved health benefits. Metabolite profiles from grape juice of well-watered and water-deficit-treated vines will be compared with quantitative data from mRNA and protein expression patterns using comprehensive bioinformatics systems to store and analyze data sets. Ultimately, these data sets will be integrated into a reliable prediction model for wine characteristics. The proposed research will greatly facilitate future gene discovery and enable improvements to be made in both production efficiency and wine quality under environmentally adverse growing conditions.
PUBLICATIONS PRODUCED AS A RESULT OF THIS RESEARCH
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(Showing: 1 - 10 of 13)
(Showing: 1 - 13 of 13)
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Cramer GR, Cushman JC, Schooley DA, Quilici D, Vincent D, Bohlman MC, Ergul A, Tattersall EAT, Tillett R, Evans J, Delacruz R, Schlauch K, Mendes P.. "Progress in bioinformatics- the challenge of integrating transcriptomic, proteomic and
metabolomic information.," Acta Horticulturae, v.689, 2005, p. 417.
Cramer GR, Ergül A, Grimplet J, Tillett RL, Tattersall EAR, Bohlman MC, Vincent D, Sonderegger J, Evans J, Osborne C, Quilici D, Schlauch KA, Schooley DA, Cushman JC.. "Transcript and metabolite profiling of grapevines exposed to gradually increasing, long-term water-deficit or isoosmotic salinity.," Functional and Integrative Genomics, v.7, 2006, p. 111.
Espinoza C, A. Vega, C. Medina, K.A. Schlauch, G.R. Cramer, and P. Arce-Johnson.. "Analysis of gene expression associated with compatible viral diseases in grapevine cultivars.," Functional and Integrative Genomics, v.7, 2006, p. 95.
Goes da Silva F, Iandolino A, Lim H, Baek JM, Leslie A, Xu J, Cook DR, and Al-Kayal F, Bohlman MC, Cushman MA, Ergul A, Figueroa R, Kabuloglu EK, Osbourne C, Rowe J, Tattersall EA, Cramer GR, Cushman JC. "Characterizing the grape transcriptome: analysis of ESTs from multiple Vitis species and
development of a compendium of gene expression during berry development.," Plant Physiology, v.139, 2005, p. 574.
Grimplet J, Deluc LG, Schlauch KA, Tillett RL, Wheatley MD, Cramer GR, Cushman JC.. "Tissue-specific mRNA expression profiling in grape berry tissues.," BMC Genomics, v.8, 2007, p. 187.
Mao C, Cushman JC, May G, Weller JW. "ESTAP-an automated system for the analysis of EST data," Bioinformatics., v.19, 2003, p. 1720.
Mao C, Cushman JC, May G, Weller JW. "ESTAP-an automated system for the analysis of EST data," Bioinformatics., v.19, 2003, p. 1720.
Tattersall EA, Grimplet J, Deluc LG, Wheatley MD, Vincent D, Osborne C, Ergül A, Lomen E, Blank RR, Schlauch KA, Cushman JC, Cramer GR.. "Transcript abundance profiles reveal larger and more complex responses of grapevine to chilling as compared to osmotic and salinity stress.," Functional and Integrative Genomics, v.7, 2007, p. 317.
Tattersall EAR, Ergul A, AlKayal F, Cushman JC, Grant R. Cramer.. "A Comparison of methods for isolating high quality RNA from leaves of grapevine.," American Society of Enology and Viticulture, v.56, 2005, p. 400.
Tattersall EAR, Ergul A, AlKayal F, DeLuc L, Cushman JC, Cramer GR. "Comparison of methods for isolating high-quality RNA from leaves of grapevine.," Amer. J. Vitic. Enol., v.56, 2005, p. 400.
Vincent D, Ergul A, Bohlman MC, Tattersall EA, Tillett R, Woosley R, Quilici DR, Joets J, Schlauch KA, Schooley DA, Cushman JC, Cramer GR. "Proteomic analysis reveals differences between Vitis vinifera L. cv. Chardonnay and Cabernet Sauvignon and their responses to water-deficit and salinity.," Journal of Experimental Botany, v.58, 2007, p. 1873.
Vincent D, M. Wheately and G.R. Cramer.. "Optimization of protein extraction for mature grape berry clusters.," Electrophoresis, v.27, 2006, p. 1853.
Vincent D, M. Wheately and G.R. Cramer.. "Optimization of protein extraction for mature grape berry clusters.," Electrophoresis, v.27, 2006, p. 1853.
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