Award Abstract #0239163 CAREER: Creating Nanostructured Gratings on Microstructures for Residual Strain/Stress Measurement in NEMS/MEMS and Traction Force Measurement in Cells
ABSTRACT
This Faculty Early Career Development (CAREER) Program project is intended to create nanostructured gratings (e.g., 1D nanograting lines and 2D nanograting dots) on micromachined devices for residual strain/stress measurements in MEMS (Microelectromechanical Systems) and NEMS (Nanoelectromechanical Systems); and, bio traction force measurements in real time and in live cells. This research project is a unique combination of MEMS, NEMS, nano-optics, biomechanics, and nanomanufacturing. This project involves several broad but highly interconnected areas of micro/nanomanufacturing research, all related to translating morphological recognition into micro/nanomechanics directly in thin films, in real time, and/or in live cells. This project aims at developing several novel nanostructured gratings and the corresponding measurement techniques based on the current emerging nanofabrication/nanomanufacturing technologies. Preliminary results show that the sensitivity of the methodology is at least three orders of magnitude higher than other techniques commonly used in residual strain/traction force measurements. The impact of the theoretical and experimental efforts would be significant. Through a careful leverage from current core competencies in MEMS/NEMS fabrication and biomedical processes, completion of this project will ensure that residual strain/stress in MEMS/NEMS structures and materials be directly imaged, and its magnitude quickly estimated, and more importantly, both direction and magnitude of small traction forces generated by different regions of a live cell be measured accurately and the maximum traction force generated under each of different regions of the cell be quantified in real time.
The investigator has chosen to focus the work on the domain of micro/nanomanufacturing science and technology for four reasons: (1) wide scope of the MEMS/NEMS and nanomanufacturing enterprise naturally fosters interdisciplinary research; (2) the investigator has a long track record of expertise and direct involvement with such systems and technologies, giving the project the benefit of a thorough understanding of the real issues, as well as invaluable insights; (3) unquestionable economic and technological importance of MEMS/NEMS and micro/nanomanufacturing is compelling, (4) the investigator's connections with MEMS/NEMS and micro/nanomanufacturing organizations will afford opportunities to transform the research findings to concrete tools. The research project's educational program will encompass both curriculum development and development of innovation teaching techniques. The investigator is involved in a Distance Learning Program that plays an important role promoting the manufacturing engineering department in the classroom and online, providing important industrial connections, international recognition, and important technological initiatives. Students can benefit greatly from opportunities to design, implement and operate complete micro/nanosystems. The investigator has recently created one and will further develop one more micro/nanomanufacturing new courses that give students these opportunities. Collaborative work is planned and broader two-way interaction between the university and industry is highly anticipate
PUBLICATIONS PRODUCED AS A RESULT OF THIS RESEARCH
Li, B.; Yu, H.; Sharon, A.; Zhang, X.; . "Rapid three-dimensional manufacturing of microfluidic structures using a scanning laser system," Applied Physics Letters, v.85, 2004, p. 2426-2428.
Li, B; Yu, H; Sharon, A; Zhang, X. "Rapid three-dimensional manufacturing of microfluidic structures using a scanning laser system," APPLIED PHYSICS LETTERS, v.85, 2004, p. 2426-2428.
Lin, I.-K.; Liao, Y.-M.; Liu,Y.; Ou, K.-S.; Chen, K.-S.; Zhang, X.; . "Viscoelastic mechanical behavior of soft microcantilever-based force sensors," Applied Physics Letters, v.93, 2008, p. 251907.
Mo Yang; Chee Chew Lim; Ronglih Liao; Xin Zhang; . "Oriented and Vectorial Patterning of Cardiac Myocytes using a Microfluidic Dielectrophoresis Chip," Journal of Microelectromechanical Systems, v.15, 2006, p. 1483-1491.
Mo Yang; Xin Zhang; . "A Novel Impedance Assay for Cardiac Myocyte Hypertrophy Sensing," Sensors and Actuators A, v.136, 2007, p. 504-509.
Mo Yang; Xin Zhang; . "Electrical Assisted Patterning of Cardiac Myocytes with Controlled Macroscopic Anisotropy using a Microfluidic Dielectrophoresis Chip," Sensors and Actuators A: Physical, v.135, 2007, p. 73-79.
Qiu, Y., Liao, R., Zhang, X.. "Impedance-Based Monitoring of Ongoing Cardiomyocyte Death Induced by Tumor Necrosis Factor Alpha," Biophysical Journal, v.96, 2009, p. 1985-1991.
Qiu, YL; Liao, RL; Zhang, X. "Real-time monitoring primary cardiomyocyte adhesion based on electrochemical impedance spectroscopy and electrical cell-substrate impedance sensing," ANALYTICAL CHEMISTRY, v.80, 2008, p. 990-996.
Wang, YP; Zheng, XYR; Riddick, N; Bryden, M; Baur, W; Zhang, X; Surks, HK. "ROCK Isoform Regulation of Myosin Phosphatase and Contractility in Vascular Smooth Muscle Cells," CIRCULATION RESEARCH, v.104, 2009, p. 531-U226.
Yang, M.; Lim, C. C.; Liao, R.; Zhang, X.; . "A novel microfluidic impedance assay for monitoring endothelin-induced cardiomyocyte hypertrophy," Biosens Bioelectron, v.22, 2007, p. 1688-1693.
Yang, M.; Lim, C.; Liao, R.; Zhang, X.; . "Oriented and vectorial patterning of cardiac myocytes using a microfluidic dielectrophoresis chip," Journal of Microelectromechanical Systems, v.15, 2006, p. 1483-1491.
Yang, M; Lim, CC; Liao, R; Zhang, X. "A novel microfluidic impedance assay for monitoring endothelin-induced cardiomyocyte hypertrophy," BIOSENSORS & BIOELECTRONICS, v.22, 2007, p. 1688-1693.
Yang, M; Zhang, X. "Electrical assisted patterning of cardiac myocytes with controlled macroscopic anisotropy using a microfluidic dielectrophoresis chip," SENSORS AND ACTUATORS A-PHYSICAL, v.135, 2007, p. 73-79.
Yang, M; Zhang, X. "A novel impedance assay for cardiac myocyte hypertrophy sensing," SENSORS AND ACTUATORS A-PHYSICAL, v.136, 2007, p. 504-509.
Yu, H.; Balogun, O.; Li, B.; Murray, T. W.; Zhang, X.; . "cation of three-dimensional microstructures based on singled-layered SU-8 for lab-on-chip applications," Sensors and Actuators a-Physical, v.127, 2006, p. 228-234.
Yu, H.; Balogun, U.; Li, B.; Murray, T. W.; Zhang, X.; . "Building embedded microchannels using a single layered SU-8, and determining Young," Journal of Micromechanics and Microengineering, v.14, 2004, p. 1576-1584.
Yu, H.; Li, B.; Zhang, X.; . "Flexible fabrication of three-dimensional multi-layered microstructures using a scanning laser system," Sensors and Actuators a-Physical, v.125, 2006, p. 553-564.
Yu, H; Balogun, O; Li, B; Murray, TW; Zhang, X. "Fabrication of three-dimensional microstructures based on singled-layered SU-8 for lab-on-chip applications," SENSORS AND ACTUATORS A-PHYSICAL, v.127, 2006, p. 228-234.
Yu, H; Balogun, U; Li, B; Murray, TW; Zhang, X. "Building embedded microchannels using a single layered SU-8, and determining Young's modulus using a laser acoustic technique," JOURNAL OF MICROMECHANICS AND MICROENGINEERING, v.14, 2004, p. 1576-1584.
Yu, H; Li, B; Zhang, X. "Flexible fabrication of three-dimensional multi-layered microstructures using a scanning laser system," SENSORS AND ACTUATORS A-PHYSICAL, v.125, 2006, p. 553-564.
Zhang, X.; Zhao, Y.; Li, B.; Ludlow, D.; . "Pumping capacity and reliability of cryogenic micro-pump for micro-satellite applications," Journal of Micromechanics and Microengineering, v.14, 2004, p. 1421-1429.
Zhao, Y.; Lim, C. C.; Sawyer, D. B.; Liao, R. L.; Zhang, X.; . "Microchip for subcellular mechanics study in living cells," Sensors and Actuators B-Chemical, v.114, 2006, p. 1108-1115.
Zhao, Y.; Lim, C. C.; Sawyer, D. B.; Liao, R. L.; Zhang, X.; . "Cellular force measurements using single-spaced polymeric microstructures: isolating cells from base substrate," Journal of Micromechanics and Microengineering, v.15, 2005, p. 1649-1656.
Zhao, Y.; Lim, C. C.; Sawyer, D. B.; Liao, R. L.; Zhang, X.; . "Simultaneous orientation and cellular force measurements in adult cardiac myocytes using three-dimensional polymeric microstructures," Cell Motility and the Cytoskeleton, v.64, 2007, p. 718-725.
Zhao, Y.; Zhang, X.; . "Nanobiotechnology: An engineering's foray into biology," Advances in Computers, Academic Press, v.71, 2007, p. 39-102.
Zhao, Y.; Zhang, X.; . "Adaptation of flexible polymer fabrication to cellular mechanics study," Applied Physics Letters, v.87, 2005, p. -.
Zhao, Y.; Zhang, X.; . "An approach for creating polymeric microstructures with various aspect ratios for cellular analysis applications," Sensors and Actuators a-Physical, v.127, 2006, p. 216-220.
Zhao, Y.; Zhang, X.; . "Determination of the deformations in polymeric nanostructures using geometric moire techniques for biological applications," Sensors and Actuators B-Chemical, v.117, 2006, p. 376-383.
Zhao, Y.; Zhang, X.; . "Nanobiotechnology: An engineer," Advances in Computers, Vol 71, v.71, 2007, p. 39-102.
Zhao, Y.; Zhang, X.; . "Cellular mechanics study in cardiac myocytes using PDMS pillars array," Sensors and Actuators a-Physical, v.125, 2006, p. 398-404.
Zhao, Y.; Zhang, X.; . "Adaptation of myofibrils to a microstructured polymeric substrate," Sensors and Actuators a-Physical, v.136, 2007, p. 491-495.
Zhao, Y; Lim, CC; Sawyer, DB; Liao, RL; Zhang, X. "Cellular force measurements using single-spaced polymeric microstructures: isolating cells from base substrate," JOURNAL OF MICROMECHANICS AND MICROENGINEERING, v.15, 2005, p. 1649-1656.
Zhao, Y; Lim, CC; Sawyer, DB; Liao, RL; Zhang, X. "Microchip for subcellular mechanics study in living cells," SENSORS AND ACTUATORS B-CHEMICAL, v.114, 2006, p. 1108-1115.
Zhao, Y; Lim, CC; Sawyer, DB; Liao, RL; Zhang, X. "Simultaneous orientation and cellular force measurements in adult cardiac myocytes using three-dimensional polymeric microstructures," CELL MOTILITY AND THE CYTOSKELETON, v.64, 2007, p. 718-725.
Zhao, Y; Zhang, X. "Adaptation of flexible polymer fabrication to cellular mechanics study," APPLIED PHYSICS LETTERS, v.87, 2005.
Zhao, Y; Zhang, X. "Cellular mechanics study in cardiac myocytes using PDMS pillars array," SENSORS AND ACTUATORS A-PHYSICAL, v.125, 2006, p. 398-404.
Zhao, Y; Zhang, X. "An approach for creating polymeric microstructures with various aspect ratios for cellular analysis applications," SENSORS AND ACTUATORS A-PHYSICAL, v.127, 2006, p. 216-220.
Zhao, Y; Zhang, X. "Determination of the deformations in polymeric nanostructures using geometric moire techniques for biological applications," SENSORS AND ACTUATORS B-CHEMICAL, v.117, 2006, p. 376-383.
Zhao, Y; Zhang, X. "Adaptation of myofibrils to a microstructured polymeric substrate," SENSORS AND ACTUATORS A-PHYSICAL, v.136, 2007, p. 491-495.
Zhao, Y; Zhang, X. "Profile control in silicon nanostructures using fluorine-enhanced oxide passivation," IEEE TRANSACTIONS ON NANOTECHNOLOGY, v.7, 2008, p. 40-47.
Zheng, XY; Zhang, X. "Optical moire as a visualization tool for living vascular cell contraction force mapping," APPLIED PHYSICS LETTERS, v.93, 2008.
Zheng, XY; Zhang, X. "An optical Moire technique for cell traction force mapping," JOURNAL OF MICROMECHANICS AND MICROENGINEERING, v.18, 2008.
