Leon Esterowitz
CBET Division of Chemical, Bioengineering, Environmental, and Transport Systems
ENG Directorate for Engineering
Start Date:
August 15, 2003
Expires:
July 31, 2007 (Estimated)
Awarded Amount to Date:
$567567
Investigator(s):
Bogdan Dragnea dragnea@indiana.edu (Principal Investigator)
C. Cheng Kao (Co-Principal Investigator)
Sponsor:
Indiana University
P O Box 1847
Bloomington, IN 47402 812/855-0516
NSF Program(s):
BIOPHOTONICS, IMAGING &SENSING, NANOSCALE: SCIENCE & ENGIN CTR
Field Application(s):
0000099 Other Applications NEC, 0203000 Health
Program Reference Code(s):
OTHR, 9232, 7234, 1606, 005E, 0000
Program Element Code(s):
V113, 7236, 1675
ABSTRACT
0322767
Dragnea
The approach proposed here is to avoid averaging over different sub-populations by studying single viruses and to obtain time-resolved structural information in a controlled physiological environment. To this end, because of the extremely small amount of sample in a single virus, utilization of spectroscopic enhancement techniques is required. Functionalized gold nanoparticles introduced into the viral cavity, will play the role of spectroscopic enhancers. In-vitro expansion of the single capsid will be induced by pH variations and monitored by two spectroscopic techniques: surface plasmon resonance spectroscopy and chemically-specific surface enhanced Raman scattering spectroscopy. The goal is to obtain a quantitative measure of the capsid expansion and the number of intermediate transitions.
PUBLICATIONS PRODUCED AS A RESULT OF THIS RESEARCH
Amarie, D; Rawlinson, ND; Schaich, WL; Dragnea, B; Jacobson, SC. "Three-dimensional mapping of the light intensity transmitted through nanoapertures," NANO LETTERS, v.5, 2005, p. 1227-1230.
Chao Chen, Eun-Soo Kwak, Barry Stein, C. Cheng Kao, Bogdan Dragnea. "Packaging of Gold Particles in Viral Capsids," J. Nanoscience and Nanotechnology, v.08, 2005, p. 01.
Chen, C., E.-S. Kwak, B. Stein, C. C. Kao, and B. Dragnea. "Packaging of Gold Particles in Viral Capsids," Journal of Nanoscience and Nanotechnology, v.5, 2005, p. 2029.
Chen, C., M.-C. Daniel, Z. Quinkert, M. De, Barry Stein, V.M. Rotello, C. C. Kao, and B. Dragnea. "High yield synthesis of virus-like particles," NanoLetters, v.6, 2006, p. 611.
Dixit, S., N. L. Goicochea, M. C. Daniel, A. Murali, L. Bronstein, M. De, B. Stein, V. M. Rotello, C. Kao, and B. Dragnea.. "Quantum Dot Encapsulation in Viral Capsids," NanoLetters, v.6, 2006, p. 1993.
Eun-Soo Kwak, Tiberiu-Dan Onuta, Dragos Amarie,, Radislav Potyrailo, Barry Stein, Stephen C. Jacobson, W. L. Schaich, and Bogdan Dragnea. "Optical Trapping with Integrated Near-Field Apertures," Journal of Physical Chemistry B, 2004, p. ASAP.
Sun, J., DuFort, C. A. Murali, M. D. Daniel, C. Chen, K. Gopinath, B. Stein, M. De, Vince Rotello, A. Holzenburg, C. C. Kao, and B. Dragnea.. "Core-controlled polymorphism in virus-like particles," Proceedings of the National Academy of Sciences USA, v.104, 2007, p. 1354.
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