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Award Abstract #0330075
SENSORS: Rapid, Multi-Analyte Immuno Biosensor with Passive Microfluidics: A Model System - Four Cardiac Marker Monitoring Device
| NSF Org: |
CBET
Division of Chemical, Bioengineering, Environmental, and Transport Systems
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| Initial Amendment Date: |
August 14, 2003 |
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| Latest Amendment Date: |
August 14, 2003 |
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| Award Number: |
0330075 |
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| Award Instrument: |
Standard Grant |
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| Program Manager: |
Leon Esterowitz
CBET Division of Chemical, Bioengineering, Environmental, and Transport Systems
ENG Directorate for Engineering
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| Start Date: |
January 1, 2004 |
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| Expires: |
December 31, 2007 (Estimated) |
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| Awarded Amount to Date: |
$686000 |
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| Investigator(s): |
Kyung Kang Kyung.Kang@louisville.edu (Principal Investigator)
Chong Ahn (Co-Principal Investigator)
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| Sponsor: |
University of Louisville Research Foundation Inc
2301 S. Third St.
Louisville, KY 40208 502/852-8367
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| NSF Program(s): |
BIOMEDICAL ENGINEERING
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| Field Application(s): |
0203000 Health
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| Program Reference Code(s): |
OTHR, 9150, 7224, 0000
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| Program Element Code(s): |
5345
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ABSTRACT
0330075
Kang
The goal of this research is to develop Antibody Sensitized Tapered Fiber Sensors arrays and Oligonucleotide Signatured Fiber Sensors for rapid direct detection and quantification of bioterrorism agents and their confirming DNA signatures using optical means with high specificity and sensitivity, and very low false positives. The proposed sensors will use evanescent absorption, scatter and fluorescence in continuous biconical tapered fibers for detection and quantification. The investigators will examine one- and multi- photon evanescent absorption, scatter and fluorescence for detection. The proposed sensors will not only measure the presence of bioterrorism agent(s) at very high sensitivity, but also establish their viability. The use of multiple strategies: evanescent absorption, scatter and fluorescence in single and multi-photon mode with the aid of surface immobilized monoclonal antibodies and unique DNA sequences specific to the target organism offers a comprehensive approach for detection.
The broader impacts of this research are several-fold. The ability to detect and identify the presence or absence of pathogens or bioterrorism agents is essential for fighting terrorism. In addition, pin pointing the nature of the pathogen, especially its viability and virulence is paramount. If these can be accomplished expeditiously at the resolution of a single bacterium, the benefits to the society could be enormous. In addition, the sensors developed under this research can also be used for metabolic imaging of tissue in vivo. By positioning the tapers external to the tissue, the penetration of the 2-photon fluorescence excitation will enable the metabolic activity information from depths of several centimeters to be determined optically. A sequence of these tapers over the breast (as an example) can identify malignant tumors several centimeters beneath the surface.
PUBLICATIONS PRODUCED AS A RESULT OF THIS RESEARCH
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(Showing: 1 - 14 of 14)
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B. Hong, L. Tang, Y. J. Ren, and K. A. Kan. "Real-time, Automated, Fluorophore Mediated Multi-Cardiac Marker Biosensing System With Nano-metallic Particle Reagent," Adv Exp Med Biol., Oxygen Transport to Tissue XXVIII, v.599, 2007, p. 23.
B. Hong, L. Tang, Y.J. Ren, and Kang, K.A.. "Real-time, Automated, Fluorophore Mediated Multi-Cardiac Marker Biosensing System With Nano-metallic Particle Reagent,," Adv Exp Med Biol., 599, Oxygen Transport to Tissue XXVIII, v.599, 2007, p. 23.
Hong, B. and Kang, K.A.. "Biosensor compatible, nanogold particle fluorescence enhancer for optical immunoassay," Biosensor and Bioelectronics, v.21, 2006, p. 1333.
Hong, B. and Kang, K.A.. "Biosensor compatible, nanogold particle fluorescence enhancer for optical immunoassay," Biosensor and Bioelectronics, v.21, 2006, p. 1333.
Hong, B., Kai, J., Ren, Y., Han, J., Zou, J. Ahn, C.H., and Kang, K.A.. "Highly Sensitive, Rapid, Reliable, and Automatic, Cardiovascular Disease Diagnosis with Nanoprticle Fluorescence Enhancer and MEMS," Oxygen Transport to Tissue XXIX: Advances in Experimental Medicine and Biology, v.614, 2008, p. 265.
Hong, B., Tang, L., and Kang, K.A.. "Fluorescence Enhancers for Fluorophore Mediated Biosensors for Cardiovascular Disease Diagnosis," Proceeding of 2004 Annual ISOTT Meeting, v.578, 2006, p. 179.
Kai, J., Zou, Z., Han, J., Lee, S. Hong, B., Ren, Y., Kang, K.A., and Ahn, C.H.. "automated fluidic system with a chaotic micriofluidic reaction chamber for rapid, multi-analyte immunosensing," Proceedings of Transducers 2007, 2007, p. 735.
L. Tang, B. Ren, Y.J., Hong, B. and Kang, K.A.. "A Fluorophore-mediated, Fiber-optic, Multi-analyte, Immuno-sensing System for Rapid Diagnosis and Prognosis of Cardiovascular Diseases," Journal of Biomedical Optics, v.11, 2006, p. 021011.
Lee, S.,.Hong, B., Kang, K.A. and Ahn, C.H.. "A Disposable Optical Polymer Lab-On-A Chip With Injection-Molded Polymer Waveguides For Measuring Protein-C," Proceedings Of The 9th World Congress On Biosensors, 2006.
Tang L., Kwon, H.J. and, Kang, K.A.. "Theoretical and Experimental Analysis of Analyte Transport in a Fiber-Optic, Protein C Immuno-biosensor," Biotechnology and Bioengineering, v.88, 2004, p. 869.
Tang, L. and Kang, K.A.. "Preliminary study of fiber optic multi-cardiac-marker biosensing system for rapid coronary heart disease diagnosis and prognosis,," Adv Exp Med Biol., Oxygen Transport to Tissue XXVII, v.578, 2006, p. 101.
Tang, L. and Kang, K.A.. "Preliminary study of simultaneous multi-anticoagulant deficiency diagnosis by fiber optic multi-analyte biosensor," Advances in Experimental Medicine and Biology - Oxygen Transport to Tissue XXVI, v.566, 1995, p. 303.
Tang, L. and Kang, K.A.. "Preliminary study of fiber optic multi-cardiac-marker biosensing system for rapid coronary heart disease diagnosis and prognosis,," Adv Exp Med Biol., 578, Oxygen Transport to Tissue XXVII, v.578, 2006, p. 101.
Tang, L. and Kang, K.A.. "Fiber Optic Biosensing System Development for Simultaneous Multi-Anticoagulant Deficiency Diagnosis and MEMS Application," Advances in Experimental Medicine and Biology, Oxygen Transport to Tissue XXVI, v.566, 2005, p. 303.
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