Diane Jofuku Okamuro
IOS Division of Integrative Organismal Systems
BIO Directorate for Biological Sciences
Start Date:
September 1, 2004
Expires:
August 31, 2010 (Estimated)
Awarded Amount to Date:
$2257232
Investigator(s):
James Birchler BirchlerJ@missouri.edu (Principal Investigator)
Kathleen Newton (Co-Principal Investigator)
Sponsor:
University of Missouri-Columbia
310 JESSE HALL
COLUMBIA, MO 65211 573/882-7560
NSF Program(s):
PLANT GENOME RESEARCH PROJECT
Field Application(s):
Program Reference Code(s):
BIOT, 9109
Program Element Code(s):
1329
ABSTRACT
The project will develop a method to visually identify each of the ten chromosomes of maize. A set of probes that localize to specific sites on each chromosome and that produce a specific pattern of fluorescence will be generated. Once this method is optimized, the variation of karyotypes (chromosome images) among diverse maize germplasm and closely related species will be determined. The types of organelle genome transfer to the nucleus in these materials will be established. The karyotyping system will be used to examine the nature of genome size variation in hybrid plants by determination of changes in quantity of repetitive sequence arrays at different locations in the genome. Primed in situ labeling coupled with polymerase chain reaction amplification will be adapted for maize chromosomes and used to test the ability to detect specific subclasses of repeated sequences as well as the visualization of individual genes and introduced genes. Chromosome painting probes will be developed from diverse sources and used to label the chromosomes with multiple colors as an aid to detection of subtle changes in chromosome structure.
The development of a robust chromosome identification system will enhance studies of maize genomics. With the ability to identify each chromosome, determinations of changes in chromosome number and structure can be easily made. Investigations that examine chromosome behavior or rearrangement will be greatly facilitated. Variations in gene structure in different lines of maize can be visualized using primed in situ polymerase chain reaction amplification. The completion of this project will reveal new knowledge about chromosome structure, organelle genome transfer, the behavior and variation of repetitive sequences and the evolution of the genome.
Access to project outcomes
The results will be presented in publications and a project website that will be linked to the MaizeGDB database.
PUBLICATIONS PRODUCED AS A RESULT OF THIS RESEARCH
Akio Kato, Patrice S. Albert, Juan M. Vega and James A. Birchler. "Sensitive FISH signal detection in maize using directly labeled probes produced by high concentration DNA polymerase nick translation.," Biotechnic & Histochemistry, v.81, 2006, p. 71.
Birchler JA. "B chromosomes in plants," McGraw-Hill Encyclopedia of Science and Technology., 2007.
Birchler Ja, Albert PS, Gao Z. "Stability of repeated sequence clusters in hybrids of maize as revealed by FISH.," Tropical Plant Biology, 2008.
Danilova, TV and Birchler JA. "Integrated cytogenetic map of mitotic metaphase chromosome 9 of maize: resolution, sensitivity and banding paint development.," Chromosoma, 2008.
Fangpu Han, Jonathan C. Lamb and James A. Birchler. "High frequency of centromere inactivation resulting in stable dicentric chromosomes of maize," Proc. Natl. Acad. Sci. USA, v.103, 2006, p. 3238.
Fangpu Han, Jonathan C. Lamb, Weichang Yu, Zhi Gao and James A. Birchler. "Centromere function and nondisjunction are independent components of the maize B
chromosome accumulation mechanism.," The Plant Cell, v.19, 2007, p. 524.
Han, FP; Gao, Z; Yu, WC; Birchler, JA. "Minichromosome analysis of chromosome pairing, disjunction, and sister chromatid cohesion in maize," PLANT CELL, v.19, 2007, p. 3853-3863.
Jianbo Zhang, Chuanhe Yu, Vinay Pulletikurti, Jonathan Lamb, Tatiana Danilova, David F. Weber, James A. Birchler and Thomas Peterson. "Alternative Ac/Ds transposition induces major chromosomal rearrangements in maize.," Genes and Development, v.23, 2009, p. 755.
Jonathan C. Lamb and James A. Birchler. "Retroelement Genome Painting: Cytological visualization of retroelement expansions in the genera Zea and Tripsacum.," Genetics, v.173, 2006, p. 1007.
Jonathan C. Lamb, Fangpu Han, Weichang Yu and James A. Birchler. "Plant chromosomes from end to end: telomeres, heterochromatin and centromeres.," Current Opinion in Plant Biology, v.10, 2007, p. 116.
Jonathan C. Lamb, Julie M. Meyer and James A. Birchler. "A hemicentric inversion in the maize line knobless Tama flint created two sites of centromeric
elements and moved the kinetochore-forming region.," Chromosoma, v.116, 2007, p. 237.
Jonathan C. Lamb, Julie M. Meyer, Blake Corcoran, Akio Kato, Fangpu Han and James A. Birchler. "Distinct chromosomal distributions of highly repetitive sequences in maize.," Chromosome Research, v.15, 2007, p. 33.
Jonathan C. Lamb, Nicole C. Riddle, Ya-ming Cheng, James Theuri and James A. Birchler. "Localization and transcription of a retrotransposon-derived element on the maize B
chromosome.," Chromosome Research, v.15, 2007, p. 33.
Lamb, JC; Riddle, NC; Cheng, YM; Theuri, J; Birchler, JA. "Localization and transcription of a retrotransposon-derived element on the maize B chromosome," CHROMOSOME RESEARCH, v.15, 2007, p. 383-398.
Lough, AN; Roark, LM; Kato, A; Ream, TS; Lamb, JC; Birchler, JA; Newton, KJ. "Mitochondrial DNA transfer to the nucleus generates extensive insertion site variation in maize," GENETICS, v.178, 2008, p. 47-55.
Matthew J. Bauer and James A. Birchler. "Organization of endoreduplicated chromosomes in the endosperm of Zea mays L," Chromosoma, v.115, 2006, p. 383.
Weichang Yu, Fangpu Han, Akio Kato and James A. Birchler. "Characterization of a maize isochromosome 8S.S.," Genome, v.49, 2006, p. 700.
Weichang Yu, Fangpu Han, Zhi Gao, Juan M. Vega and James A. Birchler. "Construction and behavior of engineered minichromosomes in maize.," PNAS, v.104, 2007, p. 8924.
Weichang Yu, Jonathan C. Lamb, Fangpu Han and James A. Birchler. "Telomere-mediated chromosomal truncation in maize," PNAS, v.103, 2006, p. 17331.
Weichang Yu, Jonathan C. Lamb, Fanpu Han and James A. Birchler. "Cytological visualization of DNA transposons and their transposition pattern in somatic cells of maize.," Genetics, v.175, 2007, p. 31.
