0000099 Other Applications NEC, 0116000 Human Subjects
Program Reference Code(s):
OTHR, 1187, 1045, 004E, 0000
Program Element Code(s):
7236, 5345
ABSTRACT
0448950
Yeh
ABSTRACT
This CAREER-development plan aims to integrate multidimensional studies at nano-, micro-, and macroscales for a systems based approach to complex biomedical problems
linking molecular cell biology and chemistry to physiological (dys)function. Integration of multidimensional studies in tissue optics, mechanobiology and optical clearing will be achieved by interfacing nonlinear optical microscopy (NLOM) with electron microscopy, genomic profiling, biomechanical stress-strain and clinical, in vivo measurements. NLOM is a non-destructive, laser scanning microscopy technique, which can image (sub-) cellular processes in a thick, living tissue specimen over the course of a biological event without fixing, staining or sectioning. Specific imaging of tissue constituents by NLOM will be accomplished via nonlinear optical spectroscopy (e.g., second-harmonic generation and two-photon excited fluorescence). NLOM will be used to monitor tissue structure, organization, composition with (sub-) cellular resolution and changes due to altered mechanical and chemical environments and growth and remodeling.
PUBLICATIONS PRODUCED AS A RESULT OF THIS RESEARCH
A.M. Larson
A.T. Yeh. "Ex vivo characterization of sub-10-fs pulses," Optics Letters, v.31, 2006, p. 1681.
A.T. Yeh
J. Hirshburg. "Molecular interactions of exogenous chemical agents with collagen--implications for tissue optical clearing," Journal of Biomedical Optics, v.11, 2006, p. 014003.
A.T. YehH. GibbsJ.-J. HuA.M. Larson. "Advances in nonlinear optical microscopy for visualizing dynamic tissue properties in culture," Tissue Engineering Part B: Reviews, v.14, 2008, p. 119.
B.K. WickerH.P HutchensQ. WuA.T. YehJ.D. Humphrey. "Normal basilar artery structure and biaxial mechanical behavior," Computer Methods in Biomechanics and Biomedical Engineering, v.11, 2008, p. 539.
Bui AK, McClure RA, Chang J, Stoianovici C, Hirshburg J, Yeh AT, Choi B. "Revisiting optical clearing with dimethyl sulfoxide (DMSO)," Lasers in Surgery & Medicine, v.41, 2009, p. 142.
J. Hirshburg
B. Choi
J.S. Nelson
A.T. Yeh. "Collagen solubility correlates with skin optical clearing," Journal of Biomedical Optics, v.11, 2006, p. 040501.
J. Hirshburg
B. Choi
J.S. Nelson
A.T. Yeh. "Correlation between collagen solubility and skin optical clearing using sugars," Lasers in Surgery and Medicine, v.39, 2007, p. 140.
J.D. HumphreyP.B. WellsS. BaekJ.-J. HuK.L. McLeroyA.T. Yeh. "A theoretically-motivated biaxial tissue culture system with intravital microscopy," Biomechanics and Modeling in Mechanobiology, v.7, 2008.
Larson, AM; Yeh, AT. "Delivery of sub-10-fs pulses for nonlinear optical microscopy by polarization-maintaining single mode optical fiber," OPTICS EXPRESS, v.16, 2008, p. 14723-14730.
Lee P-F, Yeh AT, Bayless KJ. "Nonlinear optical microscopy reveals invading endothelial cells anisotropically alter three-dimensional collagen matrices," Experimental Cell Research, v.315, 2009, p. 396.
P.B. Wells
A.T. Yeh
J.D. Humphrey. "Influence of glycerol on the mechanical behavior and thermal damage susceptibility of collagenous tissues," IEEE Transactions on Biomedical Engineering, v.53, 2006, p. 747.
Q. Wu and A.T. Yeh. "Rabbit cornea microstructure response to changes in intraocular pressure visualized using nonlinear optical microscopy," Cornea, v.27, 2008, p. 2008.
Tuchin, VV; Wang, RK; Yeh, AT. "Optical clearing of tissues and cells," JOURNAL OF BIOMEDICAL OPTICS, v.13, 2008.
