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Award Abstract #0507036
NIRT: Design of Biocompatible Nanoparticles for Probing Living Cellular Functions and Their Potential Environmental Impacts
| NSF Org: |
CBET
Division of Chemical, Bioengineering, Environmental, and Transport Systems
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| Initial Amendment Date: |
June 3, 2005 |
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| Latest Amendment Date: |
August 27, 2009 |
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| Award Number: |
0507036 |
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| Award Instrument: |
Standard Grant |
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| Program Manager: |
Leon Esterowitz
CBET Division of Chemical, Bioengineering, Environmental, and Transport Systems
ENG Directorate for Engineering
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| Start Date: |
June 15, 2005 |
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| Expires: |
May 31, 2010 (Estimated) |
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| Awarded Amount to Date: |
$1399975 |
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| Investigator(s): |
Xiaohong Nancy Xu xhxu@odu.edu (Principal Investigator)
Richard Van Duyne (Co-Principal Investigator)
Christopher Osgood (Co-Principal Investigator)
Hani Elsayed-Ali (Co-Principal Investigator)
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| Sponsor: |
Old Dominion University Research Foundation
4111 Monarch Way
NORFOLK, VA 23508 757/683-4293
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| NSF Program(s): |
ENG DIVERSITY ACTIVITIES,
BIOPHOTONICS, IMAGING &SENSING,
BIOMEDICAL ENGINEERING,
ENGINEERING RESEARCH CENTERS
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| Field Application(s): |
0000099 Other Applications NEC
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| Program Reference Code(s): |
SMET, OTHR, 9179, 9102, 7218, 1674, 014E, 005E, 004E, 0000
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| Program Element Code(s): |
7680, 7236, 5345, 1480
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ABSTRACT
0507036
Xu
This proposal was received in response to Nanoscale Science and Engineering initiative, NSF 04-043, category NIRT.
The proposed work involves the design of nanoparticle probes to study the dynamics of "smart" membrane transport and the size change of membrane pores of living bacterial cells in real time. The model system to be used is the efflux pump (MexA-MexB-OprM) in P. aeruginosa because it extrudes a wide range of structurally and functionally unrelated substrates. The primary research goals are to (1) study the dependence of membrane transport kinetics on the size (1-80 nm), shape (sphere, rod, or triangle), surface charge (negative vs. positive), and properties (hydrophobic vs. hydrophilic) of nanoparticles, (2) study the interactions and assembly of efflux pump proteins in real time in response to the presence of synthesized nanoparticles with different sizes, shapes, surface charges, and properties, and (3) study the cytotoxicity and genotoxicity of the synthesized nanoparticles in vitro and in vivo to identify biocompatible and environmentally-friendly nanoparticles for real-time probing of membrane transport in living cells. This is an interdisciplinary research project in the areas of chemistry, material science and engineering, molecular and cellular biology, and protein engineering involving 4 PIs from Old Dominion University and Northwestern University: Xu (ODU, Chemistry and Biochemistry), Elsayed-Ali (ODU, Electrical and Computer Engineering), Osgood (ODU, Biological Sciences), and Van Duyne (Northwestern, Chemistry and Materials Science). An additional PI from CEA in France (Gillet, Protein Engineering) is also involved.
PUBLICATIONS PRODUCED AS A RESULT OF THIS RESEARCH
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(Showing: 1 - 19 of 19)
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10. J. M. McMahon, Y. Wang, l. J.Sherry, R. P. Van Duyne, L. D. Marks, S. K. Gray, and G. C. Schatz. "Correlating the Structure, Optical Spectra, and Electrodynamics of Single Silver Nanocubes,," J. Phys. Chem. C., v.113, 2008, p. 2731.
13. J. Dieringer, K. Wustholz, D. Masiello, J. Camden, S. Kleinman, G. Schatz, R. Van Duyne. "Surface-Enhanced Raman Excitation Spectroscopy of a Single Rhodamine 6G Molecule," J. Am. Chem. Soc., v.131, 2009, p. 849.
9. J. P. Camden, J. A. Dieringer, Y. Wang, D. J. Masiello, L. D. Marks, G. C. Schatz, R. P. Van Duyne. "Probing Single Molecule Surface Enhanced Raman Scattering Hot Spots," J. Am. Chem. Soc., v.130, 2008, p. 12616.
A. Das, J. Zhao, G. C. Schatz, S. G. Sligar, R. P. Van Duyne. "Screening of Type I and II Drugs Binding to Human Cytochrome P450-3A4 in Nanodisc by Localized Surface Plasmon Resonance Spectroscopy," Anal. Chem, v.ASAP Ap, 2009.
A. J. Haes, S. Zou, J. Zhao, G. C. Schatz, R. P. Van Duyne. "Localized surface plasmon resonance spectroscopy near molecular resonances," J. Am. Chem. Soc., v.128, 2006, p. 10905.
E. M. Hicks, O. Lyandres, M. R. Glucksberg, R. P. Van Duyne. "Plasmonic Properties of Anchored Nanoparticles Fabricated by Reactive Ion Etching and Nanosphere Lithography," J. Phys. Chem. C, v.111, 2007, p. 4116.
G. H. Chan, J. Zhao, E. M. Hicks, G. C. Schatz, R. P. Van Duyne. "Plasmonic Properties of Copper Nanoparticles Fabricated by Nanosphere Lithography," Nano Letters, v.7, 2007, p. 1947.
G. H. Chan, J. Zhao, G. C. Schatz, R. P. Van Duyne. "Localized Surface Plasmon Resonance Spectroscopy of Triangular Aluminum Nanoparticles," J. Phys. Chem. C., v.112, 2008, p. 13958.
J. A. Dieringer, R. B. Lettan, K. A. Scheidt, R. P. Van Duyne. "A Frequency Domain Existence Proof of Single-Molecule Surface Enhanced Raman Spectroscopy," J. Am. Chem. Soc., v.129, 2007, p. 16249.
J. N. Anker, W. P. Hall, M. Lambert, P. T. Velasco, W. L. Klein, R. P. Van Duyne. "Detection and Identification of Bioanalytes with High Resolution LSPR Spectroscopy and MALDI Mass Spectrometry," J. Phys. Chem. C, v.113, 2009, p. 5891.
J. Zhao, A. Das, X. Zhang, G. C. Schatz, S. G. Sligar, R. P. Van Duyne,. "Resonance localized surface plasmon spectroscopy: low molecular weight substrate binding to cytochrome P450," J. Am. Chem. Soc., v.128, 2006, p. 11004.
J. Zhao, J. Sung, S. Zou, L. Jensen, G. C. Schatz, R. P. Van Duyne. "Interaction of Plasmon and Molecular Resonances for Rhodamine 6G Adsorbed on Silver Nanoparticles," J. Am. Chem. Soc., v.129, 2007, p. 7647.
K. A. Willets, R. P. Van Duyne. "Localized surface plasmon spectroscopy and sensing," Ann. Rev. Phys. Chem., v.58, 2007, p. 267.
K. B. Biggs, J. P. Camden, J. N. Anker, R. P. Van Duyne. "Surface-Enhanced Raman Spectroscopy of Benzenethiol Adsorbed From the Gas Phase onto Silver Film over Nanosphere Surfaces: Determination of the Sticking Probability and Detection Limit Rate," J. Phys. Chem. A, v.113, 2009, p. 4581.
K. Lee, P. Nallathamby, L. Browning, C. Osgood, X. Xu*. "In Vivo Imaging of Transport and Biocompatibility of Single Nanoparticles in Early Development of Zebrafish Embryos," ACS Nano, v.1, 2007, p. 133.
L. J. Sherry, R. Jin, C. A. Mirkin, G. C. Schatz, R. P. Van Duyne,. "Localized surface plasmon resonance spectroscopy of single silver triangular nanoprisms," Nano Lett., v.6, 2006, p. 2060.
P. Nallatmamby, K. Lee, and X. Xu*. "Design of Stable and Uniform Single Nanoparticle Photonics for In Vivo Dynamics Imaging of Nanoenvironments of Zebrafish Embryonic Fluids," ACS Nano, v.2, 2008, p. 1371.
T. Huang, P. Nallathamby, D. Gillet, X. Xu*. "Design and Synthesis of Single Nanoparticle Optical Biosensors for Imaging and Characterization of Single Receptor Molecules on Single Living Cells," Anal. Chem., v.79, 2007, p. 7708.
T. Huang, P. Nallathamby, X. Xu*. "Photostable Single-Molecule Nanoparticle Optical Biosensors For Real-Time Sensing of Single Cytokine Molecules and Their Binding Reactions," J. Am. Chem. Soc., v.130, 2008, p. 17095.
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