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Award Abstract #1237936

An Interdisciplinary Approach to Deciphering the Molecular Dialogue between the Plasma Membrane and Nucleus of Medicago truncatula

Division Of Integrative Organismal Systems
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Initial Amendment Date: August 7, 2012
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Latest Amendment Date: July 12, 2014
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Award Number: 1237936
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Award Instrument: Continuing grant
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Program Manager: Timothy Nelson
IOS Division Of Integrative Organismal Systems
BIO Direct For Biological Sciences
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Start Date: September 1, 2012
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End Date: December 31, 2015 (Estimated)
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Awarded Amount to Date: $3,384,858.00
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Investigator(s): Michael Sussman msussman@wisc.edu (Principal Investigator)
Jean-Michel Ane (Co-Principal Investigator)
Joshua Coon (Co-Principal Investigator)
Sushmita Roy (Co-Principal Investigator)
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Sponsor: University of Wisconsin-Madison
21 North Park Street
MADISON, WI 53715-1218 (608)262-3822
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Program Reference Code(s): 7577, 9109, 9179, BIOT
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Program Element Code(s): 1329


PI: Michael R. Sussman (University of Wisconsin - Madison)

CoPIs: Jean Michel Ané, Joshua J. Coon, and Sushmita Roy (University of Wisconsin - Madison)

This goal of this project is to develop and apply genomic technologies for understanding at a molecular level, how bacteria and plants communicate to create a remarkable product of evolution, the nitrogen-fixing symbiotic nodule. An important part of this conversation between the microbe and the plant is a change in the chemical composition of proteins within the cell, i.e., in the degree by which phosphate groups are added or removed to specific amino acids. Using state of the art mass spectrometers to determine precisely which proteins are being modified by phosphate, and by how much in the first hour in which the bacteria meets the plant, the project will attempt to elucidate how the plant changes its proteins in order to initiate all of the myriad changes in their metabolism and gene expression that are required for the symbiotic nodules to form. All project outcomes (e.g., data, peptides and germplasm resources) will be provided to the public free of charge and without intellectual property restrictions. Medicago transcriptome, proteome and phosphoproteome data generated in this project will be deposited and accessible to the public at long-term repositories such as National Center for Biotechnology Information (NCBI: http://www.ncbi.nlm.nih.gov/), the Legume Information Service (LIS: http://www.comparative-legumes.org/) and the PeptideAtlas (www.peptideatlas.org).

The mass spectrometric technology being developed and used in this project could be useful for understanding many different biological events, besides symbiotic nitrogen fixation. In order to provide opportunities for researchers all over the U.S. to access this sophisticated instrumentation and technology for use in their research, the project will develop and hold a one-week long Plant Proteomic Workshop. To be held each summer at the University of Wisconsin, this workshop will provide two dozen participants (graduate students, postdoctoral researchers and professors) with hands-on, in-depth use of mass spectrometers for measuring changes in the proteins from plants under study in their own labs. This workshop will greatly facilitate the more widespread accessibility and use of this technology for research in the general area of plant biology, and should greatly help this country's goal of training our future plant scientists and help them become comfortable with modern genomic "systems biology" experimental techniques.


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Ye, V.H., Gemperline, E., Venkateshwaran, M., Chen, R., Delaux, P.M., Howes-Podoll, M., Ané, J.M. and Li, L.. "MALDI mass spectrometry-assisted molecular imaging of metabolites during nitrogen fixation in the Medicago truncatula ? Sinorhizobium meliloti symbiosis.," The Plant Journal, v.75, 2013, p. 130-145. 

Venkateshwaran, M., Volkening, J., Sussman, M. and Ané, J.M.. "Symbiosis and the social network of higher plants.," Current Opinion in Plant Biology, v.16, 2012, p. 118-127. 

Venkateshwaran, M., Cosme, A., Han, L., Banba, M., Satyshur, K.A., Parniske, M., Imaizumi-Anraku, H. and Ané, J.M.. "The Recent Evolution of a Symbiotic Ion Channel in the Legume Family Altered Ion Conductance and Improved Functionality in Calcium Signaling.," The Plant Cell, v.24, 2012, p. 2528-2545. 

Rose, C.M., Venkateshwaran, M., Volkening, J., Grimsrud, P.A., Maeda, J., Bailey, D.J., Howes-Podoll, M., den Os, D., Yeun, L.H., Westphall, M., Sussman, M.R., Ané, J.M. and Coon, J.J.. "Rapid phosphoproteomic and transcriptomic changes in the rhizobia-legume symbiosis.," Molecular and Cellular Proteomics, v.11, 2012, p. 724-744. 

Zhang, N., Venkateshwaran, M., Boersma, M., Harms, A., Howes-Podoll, M., den Os, D., Ane, J.M. and Sussman, M.R.. "Metabolomic profiling reveals suppression of oxylipin biosynthesis during the early stages of legume-rhizobia symbiosis.," FEBS Letters, v.586, 2012, p. 3150-3158. 

Volkening, J., Bailey, D.J., Rose, C.M., Grimsrud, P.A., Howes-Podoll, M., Venkateshwaran, M., Westphall, M., Ané, J.M., Coon, J.J. and Sussman, M.R.. "A proteogenomic survey of the Medicago truncatula genome.," Molecular and Cellular Proteomics, v.11, 2012, p. 933-944.

Rose, C.M., Venkateshwaran, M., Volkening, J., Bailey, D.J., Westphall, M.S., Sussman, M., Coon, J.J. and Ané, J.M.. "Medicago PhosphoProtein Database: a repository for Medicago truncatula phosphoprotein data," Frontiers in Plant Sciences, v.3, 2012, p. 122. 

Jayaraman D, Gilroy S, Ané JM. , 20:104?109, 2014.. "Staying in touch: mechanical signals in plant-microbe interactions.," Current Opinion in Plant Biology, v.20, 2014, p. 104.

Gemperline E, Jayaraman D, Maeda J, Ané JM, Li L.. "Multifaceted Investigation of Metabolites During Nitrogen Fixation in Medicago via High Resolution MALDI-MS Imaging and ESI-MS," J Am Soc Mass Spectrom, v.26, 2015, p. 149.

Hebert AS, Richards AL, Bailey DJ, Ulbrich A, Coughlin EE, Westphall MS, Coon JJ.. "The one hour yeast proteome.," Mol Cell Proteomics., v.13, 2014, p. 339.

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