Year 1994 Awards
Minority Postdoctoral Research Fellowships
||Research Area/Training Plan
||NSF Award #|
|Title of Research and Training Plan|
Drosophila photoreceptor physiology and the expression of the circadian
clock protein, PERIOD"
|Dr. Emery has developed an
in vitro system using the prothoracic gland from Drosophila to study control
of circadian behavioral rhythms. This preparation cyclically produces
the protein (Per) coded by the period gene and is sensitive to light/dark
modulation, making it an ideal system for this in-depth study of the effect
of light, the principal entraining environmental cue, on Per oscillation
and the role of Per as a major endogenous clock component. The long term
goal is to determine the role of Per levels in cellular physiology and
multiple memory systems in the mammalian brain"
|There appear to be 3 specialized
memory systems in the mammalian brain capable of acquiring different kinds
of information in a more-or-less independent manner. The systems seem
to interact during the acquisition of a task. The work involves the use
of reversible lesions of the structures thought to be involved. This work
promises to lead to refinements in understanding the interactions of multiple
|"Correlation of cloned
potassium channels with endogenous cardiac current"
|This research concentrates
on the role of potassium ion channels in the cardiovascular system. The
system has been well characterized through electrophysiological studies
and will now be studied using both molecular and cellular biological techniques
in complement with voltage-clamp approaches to increase understanding
of the potassium ion channel. This research focuses on the processing
of voltage-sensitive potassium ion channels in mammalian heart. The primary
goal is to examine post- translational modifications and post-transcriptional
regulataion of cardiac potassium ion channels that may alter channel expression
and function. The second goal is to correlate cloned channel structures
with native cardiac myocyte currents.
of ligule formation"
|This research investigates
the regulation of cell polarity and its contribution to the architecture
and organogenesis of higher plants. A maize mutant named LIGULELESS1 is
being characterized at the molecular and cellular levels to determine
if formation of ligules and auricles are genetically separable. The timing
and site of action of the action of this gene will be determined using
immunohistolocalization of the protein Lg1, the gene's product, in developing
leaves and by genetic mosaic analysis.
||University Of California-Davis
|| Developmental Genetics
|"Genetic and developmental
analysis of KNOTTED1 loss-of-function alleles"
|The research analyzes the first
homeobox gene identified in plants. This gene is called knotted 1 locus
(kn1) and is thought to be required to maintain the indeterminate fate
of primordial cells and its absence may be involved in the signal for
the cell to differentiate. It will be determined what is the maize phenotype
when kn1 is lacking by studying loss-of-function alleles and mutants,
by determining the effects of viability and morphology, and identifying
the nature of the mutation.
translational regulation of the human ADP/ATP translocator isoforms in
|Cells from heart have been
chosen as the model system for this study because this tissue has the
highest concentration of respiratory proteins. The adenine nucleotide
translocator (ANT) controls the supply of ATP by catalyzing the exchange
of intramitochondrial ATP for cytoplasmic ADP through the inner mitochondrial
membrane. The presence of isoforms of ANT raise interesting questions
about their regulation and synthesis. The high energy demands of the heart
require stringent regulation of these proteins and make these proteins
of special interest. The isoforms will be isolated and their regulation
studied at the molecular level.
||University of Washington
|"Mechanisms of modulation
of the stress response in wild animals"
|This research investigates
the stress response in the survival and reproduction of animals in their
natural environments. Vertebrates respond to noxious stimuli by activating
the hypothalamic-pituitary-adrenal axis and releasing glucocorticoids.
Excess glucocorticoid production during the breeding season may compromise
the animal's ability to reproduce since glucocorticoids inhibit gonadal
function. Presumably for this reason, some birds that breed in harsh conditions
have evolved a means to modulate glucocorticoid secretion. This research
seeks to elucidate this mechanism and to determine if mammals living in
similar conditions have a similar mechanism.
||Ecological & Evolutionary
|"Conflicts of interest
in genetic relatedness in social wasps"
|The theory investigated will
be that workers themselves police the egg laying of other workers, because
it is often in the workers' genetic self-interest to allow only the queen
to lay eggs. Workers are predicted to limit the reproduction of other
workers when they are less related to the progeny of other workers than
they are to the progeny of the queen. This hypothesis is tested by looking
at worker reproduction in four species of Polistes that differ in average
relatedness levels among colony members.The use of the hybrid networks
will permit the clear demonstration that activity dependent learning does
take place in the visual cortex at the level of a single cell.
||University of California, Irvine
|"Isolation and characterization
of the axolot1 MMP-9 mRNA: possible role in limb regeneration"
|Limb regeneration in salamanders
is relatively well described at the cellular level and provides a useful
system for studying several developmental events, such as pattern formation
and cell-cell interactions. The molecular mechanisms of regeneration are
yet to be elucidated but are needed for a full understanding of regeneration.
This project focuses on the dedifferentiation of limb stump tissues which
give rise to embryonic-like cells and metalloproteinases (MMPs) are theorized
to play a role in the initiation and recruitment steps of regeneration.
These proteins will be purified as a first step in studying their expression
and role in this process.
||Baylor College of Medicine
| "High resolution structural
analysis of the prototype sindbis virus"
|The primary aim of this research
is to study the structure of a membrane containing virus using Sindbis
virus as the prototype. This will be accomplished through electron cryomicroscopy
and three-dimensional reconstruction. Because this virus is enveloped,
it affords the opportunity to study its membrane proteins, and their interactions
with each other and the core proteins. The ultimate goal of the research
is to better understand the biology of the Sindbis virus by improving
the resolution of 3D reconstructions of Sindbis virus, detecting structural
transitions of Sindbis at different functional states, and comparing the
structures of wild-type and mutant viruses to understand the basis of
||University of Illinois, Urbana
|"Effects of REM Sleep
on experience-dependent morphological differences in brain structures"
|It has been hypothesized that
sleep may be important to learning and memory. This research tests the
hypothesis that a period of rapid eye movement (REM) sleep deprivation
immediately following exposure to a complex environment, produced by elevating
ambient temperature, reduces the difference in brain morphology between
enriched condition (EC) and impoverished condition (IC) rats; whereas,
REM sleep facilitation, produced by optimizing ambient temperature immediately
after EC exposure, will increase the morphological differences between
EC and IC rats. This research seeks to overcome several limitations of
earlier research on the effects of sleep and EC-IC rearing conditions.
||Fox Chase Cancer Center
|"Stopped-flow and pulsed
hydrogen exchange studies of cytochrome c mutants designed to probe the
role of helix pairing interactions and non-native histidine ligation upon
|This research intends to further
understand the folding pathway of cytochrome c by studying cytochrome
c mutants. The structural and energetic basis for the pairing interaction
between the N and C terminal helices will be used in the study because
they are know to occur at an early stage in folding.
Minority Graduate Student Travel
||University of Missouri, Columbia
||Universite' Pierre et Marie