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Discovery
Fish aglow: Hidden colors in the sea

"There's a whole light show going on down there, and people never see it."

a green glowing fish

A green biofluorescent chain catshark (Scyliorhinus retifer).
Credit and Larger Version

January 9, 2014

With the help of blue light and special long-pass filters, scientists have uncovered more of the undersea world's secrets. A study published today describes more than 180 species of marine fishes that glow in different colors and patterns, via a process known as biofluorescence.

Scientists already knew that some marine organisms fluoresce, including corals and jellyfish, but this is the first reported evidence of widespread biofluorescence among fishes.

"There's a whole light show going on down there, and people never see it," said one of the study's principal authors, John Sparks, a curator in the American Museum of Natural History's (AMNH) Department of Ichthyology.

The findings, published in PLOS ONE, will surely lead to new investigations of the function of biofluorescence as well as research related to the evolution and diversification of marine fishes. They could also lead to the discovery of new fluorescent proteins useful in cancer, brain and other biomedical research.

Biofluorescence is a natural process in which organisms absorb light at one intensity, or wavelength, and emit it at a different, usually lower, level--seen as a different color. In the ocean, the researchers found, fishes absorb the higher energy blue light around them and emit it in glowing greens, reds and oranges.

How did the scientists make the discovery? While taking and processing images of biofluorescent coral for an NSF-funded traveling museum exhibit: Creatures of Light: Nature's Bioluminescence, Sparks and AMNH research associate David Gruber (CUNY) were amazed to see, in the background of one image, an eel glowing bright green.

To further explore the phenomenon, they enlisted the help of other researchers and embarked on a series of dive expeditions. Deep underwater near the Bahamas and later the Solomon Islands, the divers shone blue lights on the ocean floor to stimulate intense biofluorescence in fishes. To see through the obliterating veil of blue light, they wore green visors over their masks and equipped their underwater camera lenses with special long-pass filters. (The researchers note that many fishes have long-pass filters in their eyes, which would allow them to see fluorescent displays.)

With the resulting images, analyses of some 12,000 specimens the team collected over four expeditions, as well as studies after hours at public aquariums, the research team discovered that biofluorescence is common throughout the tree of life for fishes. The researchers identified biofluorescence in 16 orders, 50 families, 105 genera and more than 180 species of fishes. These include the two main fish groups: cartilaginous (sharks and rays) and bony fishes (eels, lizardfishes, gobies, flatfishes).

"We know now [biofluorescence] is considerably widespread and phenotypically variable in marine fishes," said Sparks. The findings "in essence give us a road map to do fine-scale studies within certain groups to learn more about function" of biofluorescence.

Form and function

Fish fluoresce in a wide range of patterns--from simple red/orange coloration to green eye rings to more complex, species-specific patterns of interspersed fluorescent elements on the head, jaws, fins, flank and ventrum. In some cases, the fish's entire body fluoresced, including internally. The patterns were most common and variable in fishes that had cryptic coloration, or camouflage, such as eels, gobies and lizardfishes.

It was fascinating to observe major fluorescence pattern and color differences in closely-related species that otherwise look quite similar, said Sparks. Certain closely-related species of lizardfish and gobies, for example, look almost identical under white light, but strikingly different fluorescing under the filtered blue light.

Such findings could mean that fishes use biofluorescence to communicate with other species--differentiating themselves, for example--without signaling predators. This ability could be especially useful during mating rituals under a full moon, when fish are vulnerable to predators.

New protein source?

The AMNH research opens the door to new studies that could yield new proteins for use in biomedical research.

"The discovery of green fluorescent protein in a hydrozoan jellyfish in the 1960s has provided a revolutionary tool for modern biologists, transforming our study of everything from the AIDS virus to the workings of the brain," said co-lead author Gruber. "This study suggests that fish biofluorescence might be another rich reservoir of new fluorescent proteins."

Fluorescent proteins can be injected and used to track cellular functions, neural activity and more.

The AMNH-led team, funded in part by NSF, included researchers from the University of Kansas, University of Haifa, Israel and Yale University.

Editor's Note: This Behind the Scenes article was first provided to LiveScience in partnership with the National Science Foundation.

--  Jacqueline Conciatore, (703) 292-8367 jconciat@nsf.gov

Investigators
Edyta Greer
John Sparks
David Gruber
Matthew Davis
William Smith
Carrie Manfrino
Vincent Pieribone

Related Institutions/Organizations
CUNY Baruch College
CUNY Baruch College
American Museum Natural History
University of Kansas Center for Research Inc

Related Awards
#1007747 The Hidden Fluorescent World of Coral Reefs: A Multimedia Presentation
#1040321 MRI: Development of a Remote Operated Vehicle for Studies of Deep Coral Reef Biology
#0920572 RIG: Isolation, Characterization and Evolution of Fluorescent Proteins from Indo-Pacific and Caribbean Marine Organisms
#1257555 Collaborative Research: Diversification in the Deep-Sea and the Evolution of Fangs, Bioluminescence, Hermaphroditism, and Marine Habitat Transitions
#1258141 Collaborative Research: Diversification in the Deep-Sea and the Evolution of Fangs, Bioluminescence, Hermaphroditism, and Marine Habitat Transitions

Total Grants
$1,398,286

Related Websites
Read the paper The Covert World of Fish Biofluorescence on PLOS ONE: http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0083259
Watch a related video: http://www.youtube.com/watch?v=aZ3EaLXh3O4

list of various maine fishes that are flourescent
Researchers have discovered a rich diversity of fluorescent patterns and colors in marine fishes.
Credit and Larger Version

red fluorescing scorpionfish
A red fluorescing scorpionfish (Scorpaenopsis papuensis).
Credit and Larger Version

A triplefin blennie under white light (above) and blue light (below).
A triplefin blennie under white light (above) and blue light (below).
Credit and Larger Version

Researcher David Gruber  under water
Searching for new biolfuorescent organisms.
Credit and Larger Version



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